Stellate DNA
The DNA donor molecule is the genetic blueprint for gene knock-ins in
combination with CRISPR (clustered short interspaced palindromic
repeats)-Cas9 or other nucleases. While commercial products for small
gene-size knock-ins (2 kilobases or less) are available, efficient and
scalable solutions for the integration of larger payloads carrying
several genes or entire genetic circuits are currently lacking. Stellate
DNA commercializes GatalystsTM (genome catalysts), a novel class of
DNA donors that are based on our patented innovation to use circular
single stranded DNA in gene editing. GatalystsTM enable large genome
integrations (up to 20 kilobases) to be precisely targeted and minimize
cytotoxicity in cell transfections. In addition, Stellate’s proprietary
production platform allows scalable GatalystsTM production with
milligram to gram yields and up to 100-times cost reduction when
compared to competing DNA donor products. This cost reduction
allows cell therapies like CAR-T immunotherapy to be delivered to
patients faster and cheaper than with current state-of-the-art viral
vehicles and without safety issues inherent to viruses and payload
restriction by capsid sizes. The combined benefit of both production
and use of GatalystsTM enables ultra-large DNA payload integrations in
a precise manner which is truly transformational in gene and cell
engineering.
